Mouse anti‑Xenopus CDK1 / CDC2 IgG2a Monoclonal RW-C14410-50
Antibody: CDK1 / CDC2 Mouse anti-Xenopus Monoclonal (pSer/Thr) Antibody, Application: IHC, IHC-P, IHC-Fr, IF, WB, IP, Func, Reactivity: Xenopus, Human, Mouse, Rat, Chicken, Format: Unconjugated, Unmodified, Descriptio: CDC2 antibody RW-C14410 is an unconjugated mouse monoclonal antibody to CDC2 (CDK1) (pSer/Thr) from xenopus. It is reactive with human, mouse, rat and other species. Validated for Func, IF, IHC, IP and WB., Targe: Xenopus CDK1 / CDC2, Synonym: CDK1 | CDC28A | CDC2 | CDKN1 | Cyclin-dependent kinase 1 | p34 protein kinase | p34CDC2 | CDC2a | Cell cycle controller CDC2 | Cell division protein kinase 1 | PSTAIR, Hos: Mouse, Reactivit: Xenopus, Human, Mouse, Rat, Chicken (tested or 100% immunogen sequence identity), Clonalit: IgG2a Monoclonal, Conjugation: Unconjugated, Purificatio: Purified, Modification: Unmodified, Immunoge: Xenopus p34cdc2., Epitop: pSer/Thr, Specificit: Recognizes a 34kD protein cdk1, identified as cdk1/p34cdc2 protein kinase (a catalytic subunit of Maturation Promoting Factor). Its epitope maps near the C-terminus of protein and its core is thought to be LGTPNNEV. It shows no cross-reaction with cdk2/p32 and other cyclin dependent kinases (cdks). Human, mouse, rat, chicken, Xenopus., Application: IHC
IHC - Paraffin
IHC - Frozen
Immunofluorescence
Western blot (2 µg/ml)
Immunoprecipitation
Functional Assay, Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: Suitable for use in Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot and Kinase Assay. Immunoprecipitation (Native, denatured): 2 ug/mg protein lysate using Protein A. Immunohistochemistry (formalin/paraffin, frozen): 2-4 ug/ml for 30 minutes at RT. Paraffin sections require antigen retrieval. Western Blot: 2 ug/ml for 2 hours at RT. Positive control: HeLa or NIH3T3 cells; germinal center in a tonsil or reactive lymph node., Presentatio: PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA, Storag: Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About CDK1 / CDC: Uniprot: P06493 NCBI: NM_001786 NP_001777.1