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Mouse anti‑Alpha Tubulin IgM Monoclonal RW-B9831-50
Antibody: Alpha Tubulin Mouse Monoclonal (TU-16) Antibody, Application: IHC, IHC-P, IF, WB, IP, ELISA, Reactivity: Human, Mouse, Rat, Bovine, Hamster, Pig, Chicken, Plant, Protozoa, Format: Unconjugated, Unmodified, Descriptio: Alpha Tubulin antibody RW-B9831 is an unconjugated mouse monoclonal antibody to Alpha Tubulin from human, mouse, rat and other species. Validated for ELISA, IF, IHC, IP and WB., Targe: Alpha Tubulin, Hos: Mouse, Reactivit: Human, Mouse, Rat, Bovine, Hamster, Pig, Chicken, Plant, Protozoa (tested or 100% immunogen sequence identity), Clonalit: IgM Monoclonal, Clon: TU-16, Conjugation: Unconjugated, Purificatio: Purified from ascites by precipitation methods., Modification: Unmodified, Immunoge: Tubulin, alpha., Specificit: Recognizes Tubulin, alpha under both denaturing and non-denaturing conditions. Species cross-reactivity: Human, mouse, rat, hamster, porcine, bovine, chicken, plants and protozoa., Application: IHC
IHC - Paraffin (10 µg/ml)
Immunofluorescence
Western blot
Immunoprecipitation
ELISA, Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation., Presentatio: PBS, pH 7.4, 15 mM Sodium Azide, Storag: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee.
485.00 € 485.0 EUR
Mouse anti‑Arabidopsis Actin IgM,k Monoclonal RW-B14148-0.1
Antibody: Actin Mouse anti-Arabidopsis Monoclonal (mAbGEa) Antibody, Application: IHC-P, ICC, IF, WB, ELISA, Reactivity: Arabidopsis, Human, Mouse, Rat, Bovine, Rabbit, Sheep, Xenopus, Zebrafish, Drosophila, Yeast, Fungus, Hamster-Chinese, Plant, Protozoa, Format: Unconjugated, Unmodified, Descriptio: Actins are ubiquitously expressed proteins that are a major component of the cytoskeleton. Actin is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues. It regulates motility and controls cells structure in both muscle and non-muscle cells (where it is present in the cytoplasm as well). Pan-actin antibodies can be used to identify actin expression and localization in normal and disease states. Pan-actin antibodies can be used to label myotubes, myoblasts and fibroblasts. They are useful for determining total actin levels to compare against the differential expression of specific actins in normal versus diseased tissue, such as kidney tissue in diabetes.

References: Cytoskeleton (Hoboken). 2010 Nov;67(11):729-43, PMID: 20862689; Clin Proteom 8, 15 (2011) doi:10.1186/1559-0275-8-15; Molecular & Cellular Proteomics. 2012. 11 (8): 255-271, DOI: 10.1074/mcp.M111.015099, Targe: Arabidopsis Actin, Hos: Mouse, Reactivit: Arabidopsis, Human, Mouse, Rat, Bovine, Rabbit, Sheep, Xenopus, Zebrafish, Drosophila, Yeast, Fungus, Hamster-Chinese, Plant, Protozoa (tested or 100% immunogen sequence identity), Clonalit: IgM,k Monoclonal, Clon: mAbGEa, Conjugation: Unconjugated, Purificatio: Purified IgM, Modification: Unmodified, Immunoge: Purified recombinant Arabidopsis Actin protein [UniProt# P0CJ46], Specificit: Arabidopsis Actin. Arabidopsis Actin. Reacts with Actin 1, 2, 3, 4, 7, 8, 11 and 12., Application: IHC - Paraffin (5 µg/ml)
ICC (1:20 - 1:100)
Immunofluorescence (1:20 - 1:100)
Western blot (1:25)
ELISA (1:100 - 1:2000), Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: This Actin Antibody (mAbGEa) is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and ELISA. In WB, a band is seen at ~45 kDa representing Actin. In IHC-P and ICC/IF, cytoplasmic staining is observed. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10-15 uL of the recommended dilution is used per data point., Presentatio: PBS, 0.05% Sodium Azide, Storag: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee.
460.00 € 460.0 EUR
Mouse anti‑Pig Beta Tubulin IgM Monoclonal RW-C124722-100
Antibody: Beta Tubulin Mouse anti-Pig Monoclonal Antibody, Application: IHC, IHC-P, IHC-Fr, WB, ELISA, Reactivity: Pig, Human, Mouse, Plant, Format: Unconjugated, Unmodified, Descriptio: Beta Tubulin antibody RW-C124722 is an unconjugated mouse monoclonal antibody to Beta Tubulin from pig. It is reactive with human, mouse, pig and other species. Validated for ELISA, IHC and WB., Targe: Pig Beta Tubulin, Hos: Mouse, Reactivit: Pig, Human, Mouse, Plant (tested or 100% immunogen sequence identity), Clonalit: IgM Monoclonal, Conjugation: Unconjugated, Purificatio: Ion exchange chromatography, Modification: Unmodified, Immunoge: Tubulin beta from porcine brain., Specificit: Recognizes an epitope within the N-terminal region of porcine tubulin beta. Species cross-reactivity: human, plants, mouse., Application: IHC
IHC - Paraffin
IHC - Frozen
Western blot (1 µg/ml)
ELISA, Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: Suitable for use in ELISA, Immunohistochemistry (frozen), and Western blot: 1 ug/ml., Presentatio: PBS, pH 7.4, 0.09% Sodium Azide, Storag: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee.
626.00 € 626.0 EUR
Rabbit anti‑Human CA9 / Carbonic Anhydrase IX Polyclonal RW-C83728-0.1
Antibody: CA9 / Carbonic Anhydrase IX Rabbit anti-Human Polyclonal (C-Terminus) (Biotin) Antibody, Application: IHC, IHC-P, IF, WB, Reactivity: Human, Mouse, Rat, Dog, Plant, Format: Biotin, Unmodified, Other formats: Unconjugated DY650 DY488 DY550, Descriptio: Carbonic Anhydrase IX antibody RW-C83728 is a biotin-conjugated rabbit polyclonal antibody to Carbonic Anhydrase IX (CA9) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC and WB., Targe: Human CA9 / Carbonic Anhydrase IX, Synonym: CA9 | CAIX | Carbonate dehydratase IX | Carbonic anhydrase IX | CA-IX | Carbonic dehydratase | G250 | MN | Membrane antigen MN | p54/58N | RCC-associated antigen G250 | RCC-associated protein G250 | Carbonic anhydrase 9 | PMW1 | CA IX, Hos: Rabbit, Reactivit: Human, Mouse, Rat, Dog, Plant (tested or 100% immunogen sequence identity), Clonalit: Polyclonal, Conjugation: Biotin. Also available Unconjugated or conjugated with DY650, DY488, DY550., Purificatio: Immunoaffinity purified, Modification: Unmodified, Immunoge: A synthetic peptide derived from a C-Terminus sequence of the human CA IX., Epitop: C-Terminus, Specificit: Human CA IX., Application: IHC
IHC - Paraffin
Immunofluorescence (1:1000)
Western blot
Applications tested for the base form of this product only, Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Can be used for western blot, immunofluorescence, and immunohistochemistry. It has only been tested on recombinant protein in Western blot., Presentatio: PBS, 0.05% Sodium Azide, Storag: Store at 4°C. Do not freeze., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About CA9 / Carbonic Anhydrase I: Uniprot: Q16790 NCBI: NM_001216 NP_001207.2
416.00 € 416.0 EUR
Rabbit anti‑Human CA9 / Carbonic Anhydrase IX Polyclonal RW-B273-0.1
Antibody: CA9 / Carbonic Anhydrase IX Rabbit anti-Human Polyclonal (aa400-459) Antibody, Application: IHC, IHC-P, Reactivity: Human, Mouse, Rat, Dog, Plant, Format: Unconjugated, Unmodified, Other formats: Biotin DY650 DY488 DY550, Descriptio: Carbonic Anhydrase IX antibody RW-B273 is an unconjugated rabbit polyclonal antibody to Carbonic Anhydrase IX (CA9) (aa400-459) from human. It is reactive with human, mouse, rat and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues., Targe: Human CA9 / Carbonic Anhydrase IX, Synonym: CA9 | CAIX | Carbonate dehydratase IX | Carbonic anhydrase IX | CA-IX | Carbonic dehydratase | G250 | MN | Membrane antigen MN | p54/58N | RCC-associated antigen G250 | RCC-associated protein G250 | Carbonic anhydrase 9 | PMW1 | CA IX, Hos: Rabbit, Reactivit: Human, Mouse, Rat, Dog, Plant (tested or 100% immunogen sequence identity), Clonalit: Polyclonal, Conjugation: Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550., Purificatio: Immunoaffinity purified, Modification: Unmodified, Immunoge: A synthetic peptide derived from a C-Terminus sequence of the human CA IX with amino acids 400-459., Epitop: aa400-459, Specificit: Human CA IX (CA9)., Application: IHC
IHC - Paraffin (0.63 - 2.5 µg/ml), Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: Immunohistochemistry: RW-B273 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for RW-B273 was determined to be 5 ug/ml., Presentatio: PBS, 0.025% Sodium Azide, Storag: Store at -20°C. Avoid freeze-thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About CA9 / Carbonic Anhydrase I: Uniprot: Q16790 NCBI: NM_001216 NP_001207.2
460.00 € 460.0 EUR