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Mouse anti‑Human ACTB / Beta Actin IgG2b Monoclonal RW-C359268-0.1
Antibody: ACTB / Beta Actin Mouse anti-Human Monoclonal (N-Terminus) (8H10D10) Antibody, Application: ICC, IF, WB, Flo, ELISA, Reactivity: Human, Mouse, Rat, Hamster-Chinese, Primate, Format: Unconjugated, Unmodified, Descriptio: Beta Actin antibody RW-C359268 is an unconjugated mouse monoclonal antibody to Beta Actin (ACTB) (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, ICC, IF and WB., Targe: Human ACTB / Beta Actin, Synonym: ACTB | Actin, cytoplasmic 1 | Beta-actin | Beta actin | BRWS1 | Actin, beta | Beta cytoskeletal actin | PS1TP5-binding protein 1 | PS1TP5BP1, Hos: Mouse, Reactivit: Human, Mouse, Rat, Hamster-Chinese, Primate (tested or 100% immunogen sequence identity), Clonalit: IgG2b Monoclonal, Clon: 8H10D10, Conjugation: Unconjugated, Purificatio: Ammonium sulfate precipitation, Modification: Unmodified, Immunoge: Synthetic peptide corresponding to N-Terminus residues of human Beta Actin, conjugated to KLH. [UniProt# P60709], Epitop: N-Terminus, Specificit: Human ACTB / Beta Actin. Human ACTB / Beta Actin, Application: ICC (1:200 - 1:1000)
Immunofluorescence (1:200 - 1:1000)
Western blot
Flow Cytometry (1:200 - 1:400)
ELISA (1:10000), Usag: This Beta Actin (8H10D10) antibody is useful in Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence and ELISA.In Simple Western only 10-15 uL of the recommended dilution is used per data point., Presentatio: PBS, 0.03% Sodium Azide, Storag: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About ACTB / Beta Acti: Uniprot: P60709 NCBI: NM_001101 NP_001092.1
386.00 € 386.0 EUR
Rabbit anti‑Human AIMP1 / EMAP II Polyclonal RW-C359396-0.1
Antibody: AIMP1 / EMAP II Rabbit anti-Human Polyclonal (aa260-310) Antibody, Application: WB, Reactivity: Human, Mouse, Rat, Bovine, Guinea pig, Horse, Pig, Hamster-Chinese, Primate, Format: Unconjugated, Unmodified, Descriptio: EMAP II antibody RW-C359396 is an unconjugated rabbit polyclonal antibody to EMAP II (AIMP1) (aa260-310) from human. It is reactive with human, mouse, rat and other species. Validated for WB., Targe: Human AIMP1 / EMAP II, Synonym: AIMP1 | EMAP II | EMAPII | EMAP-2 | HLD3 | p43 | EMAP2 | SCYE1, Hos: Rabbit, Reactivit: Human, Mouse, Rat, Bovine, Guinea pig, Horse, Pig, Hamster-Chinese, Primate (tested or 100% immunogen sequence identity), Clonalit: Polyclonal, Conjugation: Unconjugated, Purificatio: Protein A purified, Modification: Unmodified, Immunoge: A portion of amino acids 260-310 from human AIMP1., Epitop: aa260-310, Specificit: Human AIMP1 / EMAP II, Application: Western blot (1:50), Usag: In Simple Western only 10-15 uL of the recommended dilution is used per data point., Presentatio: PBS, 0.05% Sodium Azide, 0.05% BSA, Storag: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About AIMP1 / EMAP I: Uniprot: Q12904 NCBI: NM_004757 NP_004748.2
386.00 € 386.0 EUR
Rabbit anti‑Human AIMP1 / EMAP II Polyclonal RW-C359427-0.1
Antibody: AIMP1 / EMAP II Rabbit anti-Human Polyclonal (aa720-800) Antibody, Application: IHC, IHC-P, WB, Reactivity: Human, Mouse, Rat, Bovine, Cat, Dog, Guinea pig, Horse, Opossum, Pig, Hamster-Chinese, Primate, Format: Unconjugated, Unmodified, Descriptio: EMAP II antibody RW-C359427 is an unconjugated rabbit polyclonal antibody to EMAP II (AIMP1) (aa720-800) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB., Targe: Human AIMP1 / EMAP II, Synonym: AIMP1 | EMAP II | EMAPII | EMAP-2 | HLD3 | p43 | EMAP2 | SCYE1, Hos: Rabbit, Reactivit: Human, Mouse, Rat, Bovine, Cat, Dog, Guinea pig, Horse, Opossum, Pig, Hamster-Chinese, Primate (tested or 100% immunogen sequence identity), Clonalit: Polyclonal, Conjugation: Unconjugated, Purificatio: Protein A purified, Modification: Unmodified, Immunoge: A portion of amino acids 720-800 from human AIMP1., Epitop: aa720-800, Specificit: Human AIMP1 / EMAP II, Application: IHC
IHC - Paraffin (5 µg/ml)
Western blot (2 - 4 µg/ml), Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Presentatio: PBS, 0.05% Sodium Azide, 0.05% BSA, Storag: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About AIMP1 / EMAP I: Uniprot: Q12904 NCBI: NM_004757 NP_004748.2
386.00 € 386.0 EUR
Mouse anti‑Arabidopsis Actin IgM,k Monoclonal RW-B14148-0.1
Antibody: Actin Mouse anti-Arabidopsis Monoclonal (mAbGEa) Antibody, Application: IHC-P, ICC, IF, WB, ELISA, Reactivity: Arabidopsis, Human, Mouse, Rat, Bovine, Rabbit, Sheep, Xenopus, Zebrafish, Drosophila, Yeast, Fungus, Hamster-Chinese, Plant, Protozoa, Format: Unconjugated, Unmodified, Descriptio: Actins are ubiquitously expressed proteins that are a major component of the cytoskeleton. Actin is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues. It regulates motility and controls cells structure in both muscle and non-muscle cells (where it is present in the cytoplasm as well). Pan-actin antibodies can be used to identify actin expression and localization in normal and disease states. Pan-actin antibodies can be used to label myotubes, myoblasts and fibroblasts. They are useful for determining total actin levels to compare against the differential expression of specific actins in normal versus diseased tissue, such as kidney tissue in diabetes.

References: Cytoskeleton (Hoboken). 2010 Nov;67(11):729-43, PMID: 20862689; Clin Proteom 8, 15 (2011) doi:10.1186/1559-0275-8-15; Molecular & Cellular Proteomics. 2012. 11 (8): 255-271, DOI: 10.1074/mcp.M111.015099, Targe: Arabidopsis Actin, Hos: Mouse, Reactivit: Arabidopsis, Human, Mouse, Rat, Bovine, Rabbit, Sheep, Xenopus, Zebrafish, Drosophila, Yeast, Fungus, Hamster-Chinese, Plant, Protozoa (tested or 100% immunogen sequence identity), Clonalit: IgM,k Monoclonal, Clon: mAbGEa, Conjugation: Unconjugated, Purificatio: Purified IgM, Modification: Unmodified, Immunoge: Purified recombinant Arabidopsis Actin protein [UniProt# P0CJ46], Specificit: Arabidopsis Actin. Arabidopsis Actin. Reacts with Actin 1, 2, 3, 4, 7, 8, 11 and 12., Application: IHC - Paraffin (5 µg/ml)
ICC (1:20 - 1:100)
Immunofluorescence (1:20 - 1:100)
Western blot (1:25)
ELISA (1:100 - 1:2000), Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: This Actin Antibody (mAbGEa) is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and ELISA. In WB, a band is seen at ~45 kDa representing Actin. In IHC-P and ICC/IF, cytoplasmic staining is observed. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10-15 uL of the recommended dilution is used per data point., Presentatio: PBS, 0.05% Sodium Azide, Storag: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee.
460.00 € 460.0 EUR