IHC/IF - paraffin embedded
Day 8 Mouse ovary
The antibody produces clear staining (green) in the granulosa cells of follicles. Both positive and control sections were also stained with DAPI (blue). Scale bars indicate 200μm.
Experimental protocol: Antigen retrieval: 4x 5 min at high power in an 800 W microwave in 0.01 M citrate buffer at pH 6.0, followed by a cooling period of 20 minSecondary antibody used: Alexa Fluor 488 anti-goat from Life Technologies (A11055)