Anti-CHD1L antibody [EPR14515(2)] (ab197019)
Jan 30 2019
Abcam anti-CHD1L RabMab ab197019 1-10000
antYbuddY rating:
Review Details
Company
Abcam
Product Code
ab197019
Research Area
Genome stability & stress signaling
Species Reactivity
Human (Mouse, Human according to literature)
Clonality
EPR14515(2)
Batch Number
N/A
Experiment Type
Western blot, fluorescent LICOR detection
Antibody Concentration
1:10000
Antibody Diluent
5% milk/TBS/0.1% Tween 20
Incubation Period
Overnight
Incubation Temperature
4C
Material
~20ug whole HEK293FT cell lysate (+/- transfected plasmid)
Knockdown
false
Isotype
IgG1
Experiment Year
2017
Figure Legend
PAGEruler NIR standard. Samples; lane 1, untransfected; lane 2, mock transfected; lane 3, transfected FLAG-tagged human CHD1L.
Further Information
Good antibody , giving a clear band of native protein just above 100 kDa. Faint minor bands below target band. Transfected CHD1L clearly detected. Additional lower intensity bands observed at 130kDa, not observed with other CHD1L antibodies tested.
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