Anti-CHD1L antibody [EPR14515(2)] (ab197019)

Jan 30 2019
by Chris Cooper Register or Log in to message the reviewer onsite.

Abcam anti-CHD1L RabMab ab197019 1-10000

antYbuddY rating:

Review Details

Company

Abcam

Product Code

ab197019

Research Area

Genome stability & stress signaling

Species Reactivity

Human (Mouse, Human according to literature)

Clonality

EPR14515(2)

Batch Number

N/A

Experiment Type

Western blot, fluorescent LICOR detection

Antibody Concentration

1:10000

Antibody Diluent

5% milk/TBS/0.1% Tween 20

Incubation Period

Overnight

Incubation Temperature

4C

Material

~20ug whole HEK293FT cell lysate (+/- transfected plasmid)

Knockdown

false

Isotype

IgG1

Experiment Year

2017

Figure Legend

PAGEruler NIR standard. Samples; lane 1, untransfected; lane 2, mock transfected; lane 3, transfected FLAG-tagged human CHD1L.

Further Information

Good antibody , giving a clear band of native protein just above 100 kDa. Faint minor bands below target band. Transfected CHD1L clearly detected. Additional lower intensity bands observed at 130kDa, not observed with other CHD1L antibodies tested.