Anti-CHD1L antibody [2170C3a] (ab51324)

Jan 30 2019
by Chris Cooper Register or Log in to message the reviewer onsite.

Abcam anti-CHD1L mouse ab51324 1-5000

antYbuddY rating:

Review Details

Company

Abcam

Product Code

ab51324

Research Area

Genome stability & stress signaling

Species Reactivity

Human (Mouse, Human according to literature)

Clonality

2170C3a

Batch Number

N/A

Experiment Type

Western blot, fluorescent LICOR detection

Antibody Concentration

1:5000

Antibody Diluent

5% milk/TBS/0.1% Tween 20

Incubation Period

Overnight

Incubation Temperature

4C

Material

~20ug whole HEK293FT cell lysate (+/- transfected plasmid)

Knockdown

false

Isotype

IgG1

Experiment Year

2017

Figure Legend

PAGEruler NIR standard. Samples; lane 1, untransfected; lane 2, mock transfected; lane 3, transfected FLAG-tagged human CHD1L.

Further Information

Good antibody , giving a clear band of native protein just above 100 kDa. 2 faint minor bands below target band. Transfected CHD1L clearly detected, significant additional bands below target band but presumably degradation due to overexpression.

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