Anti-CHD1L antibody [2170C3a] (ab51324)
Jan 30 2019
Abcam anti-CHD1L mouse ab51324 1-5000
antYbuddY rating:
Review Details
Company
Abcam
Product Code
ab51324
Research Area
Genome stability & stress signaling
Species Reactivity
Human (Mouse, Human according to literature)
Clonality
2170C3a
Batch Number
N/A
Experiment Type
Western blot, fluorescent LICOR detection
Antibody Concentration
1:5000
Antibody Diluent
5% milk/TBS/0.1% Tween 20
Incubation Period
Overnight
Incubation Temperature
4C
Material
~20ug whole HEK293FT cell lysate (+/- transfected plasmid)
Knockdown
false
Isotype
IgG1
Experiment Year
2017
Figure Legend
PAGEruler NIR standard. Samples; lane 1, untransfected; lane 2, mock transfected; lane 3, transfected FLAG-tagged human CHD1L.
Further Information
Good antibody , giving a clear band of native protein just above 100 kDa. 2 faint minor bands below target band. Transfected CHD1L clearly detected, significant additional bands below target band but presumably degradation due to overexpression.
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