Optineurin (C-2): sc-166576Sep 11 2018
Apoptosis, Cell death & Autophagy
1 in 50, 1 in 100, 1in 200
PBS 0.01% Triton 0.3% Fish Skin Gelatin
NSC-34 cells were cultured and fixed in methanol before immunofluorescence protocol was conducted. OPTN primary antibody was applied at 1:50, 1:100 or 1:200. Images were taken on confocal microscope.
The antibody worked well at all concentrations and could easily be used at 1:200.