Immunocytochemistry (IC or IF)
Ease of use
Glioblastoma stem cells stained with DAPI (blue) and expressing the putative stem cell marker CD133 (red)
Great antibody for IF - worked well. Is a bit expensive though and couldn't get this to work for Western blots - would be nice if Abcam gave a sample protocol for Western blot for this antibody since samples can't be used with any denaturing conditions.