Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224)

Jan 27 2019
by Chris Cooper Register or Log in to message the reviewer onsite.

Sigma anti-actin ab8224 1-40000.png

antYbuddY rating:

Review Details

Antibody name

Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224)

Research area

Genome stability & stress signaling

Company

Abcam

Product code

ab8224

Batch

N/A

Antibody application

Western Blot (WB)

Year

2019

Sensitivity

Specificity

Price value

Ease of use

Followed instructions

Yes

Friendly protocol

Results quality

Reproducibility

Product rating

Figure Legend

~20ug whole HEK293FT cell lysate loaded; antibody (1/40000) used in 5% milk/TBS/0.1-Tween 20 overnight at 4C. Fluorescent detection on LICOR system using anti-mouse secondary antibody (1/20000); PAGEruler standard. Samples; lane 1, untransfected; lane 2, mock transfected; lane 3, transfected FLAG-tagged human CHD1L.

Further Information

Excellent antibody and very sensitive, giving a clear band just above 43 kDa. Very minor bands below target actin band, presumably degradation. Can be reused at least once following freezing.

Advancing Scientific Discovery for Over 65 Years

Related Services