Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224)

Jan 27 2019
by Chris Cooper Register or Log in to message the reviewer onsite.

Sigma anti-actin ab8224 1-40000.png

antYbuddY rating:

Review Details

Antibody name

Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224)

Research area

Genome stability & stress signaling

Company

Abcam

Product code

ab8224

Batch

N/A

Antibody application

Western Blot (WB)

Year

2019

Sensitivity

Specificity

Price value

Ease of use

Followed instructions

Yes

Friendly protocol

Results quality

Reproducibility

Product rating

Figure Legend

~20ug whole HEK293FT cell lysate loaded; antibody (1/40000) used in 5% milk/TBS/0.1-Tween 20 overnight at 4C. Fluorescent detection on LICOR system using anti-mouse secondary antibody (1/20000); PAGEruler standard. Samples; lane 1, untransfected; lane 2, mock transfected; lane 3, transfected FLAG-tagged human CHD1L.

Further Information

Excellent antibody and very sensitive, giving a clear band just above 43 kDa. Very minor bands below target actin band, presumably degradation. Can be reused at least once following freezing.