Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224)Jan 27 2019
Sigma anti-actin ab8224 1-40000.png
Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224)
Genome stability & stress signaling
Western Blot (WB)
Ease of use
~20ug whole HEK293FT cell lysate loaded; antibody (1/40000) used in 5% milk/TBS/0.1-Tween 20 overnight at 4C. Fluorescent detection on LICOR system using anti-mouse secondary antibody (1/20000); PAGEruler standard. Samples; lane 1, untransfected; lane 2, mock transfected; lane 3, transfected FLAG-tagged human CHD1L.
Excellent antibody and very sensitive, giving a clear band just above 43 kDa. Very minor bands below target actin band, presumably degradation. Can be reused at least once following freezing.