Monoclonal ANTI-FLAGĀ® M2

Jan 27 2019
by Chris Cooper Register or Log in to message the reviewer onsite.

Sigma anti-FLAG F3165 1-20000

antYbuddY rating:

Review Details

Antibody name

Monoclonal ANTI-FLAGĀ® M2

Research area

Genome stability & stress signaling

Company

Sigma

Product code

F3165

Batch

N/A

Antibody application

Western Blot (WB)

Year

2019

Sensitivity

Specificity

Price value

Ease of use

Followed instructions

Yes

Friendly protocol

Results quality

Reproducibility

Product rating

Figure Legend

~20ug whole HEK293FT cell lysate loaded; antibody (1/20000) used in 5% milk/TBS/0.1-Tween 20 overnight at 4C. Fluorescent detection on LICOR system using anti-mouse secondary antibody (1/20000); PAGEruler standard. Samples; lane 1, untransfected; lane 2, mock transfected; lane 3, transfected FLAG-tagged human CHD1L (~100 kDa).

Further Information

Excellent antibody. Can be reused at least once following freezing.